Her father was Rodrigo Flores de Valdez Carballo, and her mother was Maria Isabel de Salazar y Treviño.
She married Diego de Ayala Treviño, son of Jose de Treviño and Leonor de Ayala Valverde.
Juana Francisca Ayala y Ovalle. She married Diego Gutierrez de Castro.
Diego Ayala y Ovalle. He married Inez Rodriguez de Montemayor, daughter of Miguel de Montemayor and Monica Rodriguez Treviño.
Ana Ayala y Ovalle. She requested a dispensation to marry Marcos Flores de Abrego.
Leonor Ayala y Ovalle. She married Felipe de Sosa.
Ramona (Reimunda) Ayala y Ovalle. She married Juan Francisco Rodriguez de Montemayor, son of Miguel de Montemayor and Monica Rodriguez Treviño.
Maria Ayala y Ovalle. She married Nicolas Lopez Prieto.